Vale lab Covers

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  • (pdf) - Esmaeeli, N.S., Madou, M.R., Sirajuddin, M., Fregeau, B., McKnight, D., Lexa, K., Strober, J. Spaeth, C. Hallinan, B.E., Smaoui, N., Pappas, J.G., Burrow, T.A., McDonald, M.T., Latibashvili, M., Leshinsky-Silver, E., Lev, D., Blumkin, L., Vale, R.D., Barkovich, A.J., Sherr, E.H. (2015) De novo mutations in KlF1A cause progressive encephalopathy and brain atrophy. Ann Clin Transl Neurol 2: 623-635. PMCID: PMC4479523.
  • (pdf) - Ham, H., Huynh, W., Schoon, R.A., Vale, R.D., Billadeau, D.D. (2015) HkRP3 is a microtubule-binding protein regulating lytic granule clustering and NK cell killing. J Immunol 194: 3984-3996. PMCID: PMC4390494.
  • (pdf) - Jonsson, E., Yamada, M., Vale, R.D., Goshima, G. (2015) Clustering of a kinesin-14 motor enables processive retrograde microtubule-based transport in plants. Nat Plants 1: 1-7. PMCID in process.
  • (pdf) - Petry, S., Vale, R.D. (2015) Microtubule nucleation at the centrosome and beyond. Nat Cell Biol 17: 1089-1093. (Review)
  • (pdf) - Tanenbaum, M.E., Stern-Ginossar, N., Weissman, J.S., Vale, R.D. (2015) Regulation of mRNA translation during mitosis. Elife Aug 24 (4): 19 pages. PMCID: PMC4548207.
  • (pdf) - Yildiz, A., Vale, R.D. (2015) Total internal reflection fluorescence microscopy. Cold Spring Harb. Protoc. Sep 1 2015 (9): pdb.top086348.
  • (pdf) - Yildiz, A., Vale, R.D. (2015) Tracking movements of the microtubule motors kinesin and dynein using total internal reflection fluorescence microscopy. Cold Spring Harb. Protoc. Sep 1 2015 (9): pdb.top086355.


  • (pdf) - Bhabha, G., Cheng, H.C., Zhang, N., Moeller, A., Liao, M., Speir, J.A., Cheng, Y., and Vale, R.D. (2014) Allosteric communication in the Dynein motor domain. Cell 159: 857-868. PMCID: PMC4269335.
  • (pdf) - Edelstein, A.D., Tsuchida, M.A., Amodaj, N., Pinkard, H., Vale, R.D., and Stuurman, N. (2014) Advanced methods of microscope control using MicroManager software. J Biol Methods 1: 1-10. PMCID: PMC4297649.
  • (pdf) - Hendershott, M.C. and Vale, R.D. (2014) Regulation of microtubule minus-end dynamics by CAMSAPs and Patronin. Proc Natl Acad Sci USA 111: 5860-5865. PMCID: PMC4000804.
  • (pdf) - Hui, E. and Vale, R.D. (2014) In vitro membrane reconstitution of the T-cell receptor proximal signaling network. Nat Struct Mol Biol 21: 133-142. PMCID: PMC4062301.
  • (pdf) - McKenney, R.J., Huynh, W., Tanenbaum, M.E., Bhabha, G. and Vale, R.D. (2014) Activation of cytoplasmic dynein motility by dynactin-cargo adapter complexes. Science 345: 337-341. NIHMS639978. PMCID: PMC4224444.
  • (pdf) - Ribeiro, S.A., D'Ambrosio, M.V., and Vale, R.D. (2014) Induction of focal adhesions and motility in Drosophia S2 cells. Mol. Biol. Cell 25: 3861-3869. PMCID: PMC4244196.
  • (pdf) - Schroeder, C.M., Ostrem, J.M.L., Hertz, N.T. and Vale, R.D. (2014) A Ras-like domain in the light intermediate chain bridges the dynein motor to a cargo-binding region. eLife 3: e03351. PMCID: PMC4359372.
  • (pdf) - Sirajuddin, M., Rice, L.M., and Vale, R.D. (2014) Regulation of microtubule motors by tubulin isotypes and post-translational modifications. Nat Cell Biol 16: 335-344. PMCID: PMC4117587.
  • (pdf) - Tanenbaum, M.E., Gilbert, L.A., Qi, L.S., Weissman, J.S. and Vale, R.D. (2014) A protein-tagging system for signal amplification in gene expression and fluorescence imagiing. Cell 159: 635-646. PMCID: PMC4252608.
  • (pdf) - Toret, C.C., D'Ambrosio, M.V., Vale, R.D., Simon, M.A., Nelson, W.J. (2014) A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell-cell adhesion. J Cell Biol 204: 265-279. PMCID: PMC3897182.
  • (pdf) -Vale, R.D. (2014) Preface: the role of reconstitution in cytoskeleton research. Meth Enzymol 540: xix-xxiii.


  • (pdf) - Doyle, K.M. and Vale, R.D. (2013) Creating opportunities for science PhDs to pursue careers n high school education. Mol Biol Cell 24: 3292-3296. PMCID: PMC3814146.
  • (pdf) - Moutinho-Pereira, S., Stuurman, N., Afonso, O., Hornsvelt, M., Aguiar, P., Goshima, G., Vale, R.D., Maiato, H. (2013) Genes involved in centrosome-independent mitotic spindle assembly in Drosophila S2 cells. Proc Natl Acad Sci USA 110: 19800-19013. PMCID: PMC3856817.
  • (pdf) - Petry, S., Groen, A.C., Ishihara, K., Mitchison, T.J., Vale, R.D. (2013) Branching microtubule nucleation in Xenopus egg extracts mediated by augmin and TPX2. Cell 1523: 768-777. PMCID: PMC3680348.
  • (pdf) - Tanenbaum, M.E., Vale, R.D, and McKenney, R.J. (2013) Cytoplasmic dynein crosslinks and slides anti-parallel microtubules using its two motor domains. eLife 2: 1-20. PMCID: PMC3762337.
  • (pdf) - Vale, R.D. (2013) The value of asking questions. Mol Biol Cell 24: 680-682. PMCID: PMC3596240.


  • (pdf) - Cho, C. and Vale, R.D. (2012) The mechanism of dynein motility: Insight from crystal structures of the motor domain. Biochem Biophys Acta 1823: 182-191. PMCID: PMC3249483.
  • (pdf) - Laan, L., Pavin, N., Husson, J., Romet-Lemonne, G., van Dujin, M., Lopez, M.P., Vale, R.D.Julicher, F., Reck-Peterson, S.L., Dogterom, M. (2012) Cortical dynein controls microtubule dynamics to generate pulling forces that position microtubule asters. Cell 148: 502-514. PMCID: PMC3292199.
  • (pdf) - Li, W., Zou, W., Yang, Y., Chai, Y., Chen, B., Cheng, S., Tian, D., Wang, X., Vale, R.D., Ou, G. (2012) Autophagy genes function sequentially to promote apoptotic cell corpse degradation in the engulfing cell. J Cell Biol 197: 27-35. PMCID: PMC3317810.
  • (pdf) - James, J.R. and Vale, R.D. (2012) Biophysical mechanism of T-cell receptor triggering in a reconstituted system.  Nature 487: 64-69. PMCID3393772.
  • (pdf) - Vale, R.D. (2012) Evaluating how we evaluate. Mol Biol Cell 23: 3285-3289. PMCID:PMC3431935.
  • (pdf) - Vale, R.D. (2012) How lucky can one be? A perspective from a young scientist at the right place at the right time. Nat. Med. 18: 1486-1488.
  • (pdf) - Vale, R.D., DeRisi, J., Phillips, R., Mullins, R.D., Waterman, Clare, Mitchison, T.J. (2012) Interdisciplinary graduate training in teaching labs. Science 338: 1542-1543. PMCID: PMC3810400.
  • (pdf) - Zou, W., Wang, X., Vale, R.D., Ou, G. (2012) Autophagy genes promote apoptotic cell corpse clearance. Autophagy 8: 1267-1268. NIHMSID #495002. [Commentary].


  • (pdf) - Carter, A.P., Cho, C., Jin, L., Vale, R.D. (2011) Crystal structure of the dynein motor domain. Science 331: 1159-1165. (cover). PMCID: PMC21330489.
  • (pdf) - Huckaba, T.M., Gennerich, A., Wilhelm, J.E., Chishti, A.H. and Vale, R.D. (2011) Kinesin-73 is a processive motor that localizes to Rab5-containing organelles. J Biol Chem 286: 7457-7467. PMCID: PMC3045001.
  • (pdf) - Petry, S., Pugieux, C., Nedelec, F.J., Vale, R.D. (2011) Augmin promotes meiotic spindle formation and bipolarity in Xenopus egg extracts. Proc Natl Acad Sci 108: 14473-14478. PMCID: PMC3167534.
  • (pdf) - Petry, S. and Vale, R.D. (2011) A new cap for kinetochore fibre minus ends. Nat Cell Biol. 13: 1406-1414. PMCID: PMC3532025.
  • (pdf) - Singhvi, A., Geuliere, J., Talavera, K., Cordes, S., Ou, G., Vale, R.D., Prasad, B.C., Clark, S.G. Garriga, G. (2011). The Arf GAP CNT-2 regulates the apoptotic fate in C. elegans asymmetric neuroblast divisions. Curr Biol 21: 948-954. PMCID: PMC3109113.


  • (pdf) - Carter, A.P. and Vale, R.D. (2010) Communication between the AAA+ ring and microtubule- binding domain of dynein. Biochem Cell Biol 88: 15-21. PMCID: PMC2894566.
  • (pdf) - D'Ambrosio, M.V. and Vale, R.D. (2010) A whole genome RNAi screen of Drosophila S2 cell spreading performed using automated computational image analysis. J. Cell Biol. 191: 471-478. PMCID: PMC3003316.
  • (pdf) - Edelstein, A., Amodaj, N., Hoover, K., Vale, R., and Stuurman, N. (2010) Computer control of microscopes using mManager. Curr Protocols in Molec Biol Supp 92: 14.20.2-14.20.17. NIHMS666048. (PMCID pending)
  • (pdf) - Goodwin, S.S. and Vale, R.D. (2010) Patronin regulates the microtubule network by protecting microtubule minus ends. Cell 143: 263-274. PMCID: PMC3008421.
  • (pdf) - Ou, G., Stuurman, N., D'Ambrosio, M., and Vale, R.D. (2010) Polarized myosin produces unequal-size daughters during asymmetric cell division. Science 330: 677. PMID 20929735. PMCID: PMC3032534.
  • (pdf) - Tole, S., Vale, R.D. (2010) Young leaders for biology in India. Science 329: 1441.
  • (pdf) - Uehara, R., Goshima, G., Mabuchi, I., Vale, R.D., Spudich, J.A., Griffis, E.R. (2010). Determinants of myosin II cortical localizaion during cytokinesis. Curr. Biol. 20: 1080-1085. PMCID: PMC2930192.
  • (pdf) - Vale, R.D. (2010) It's a wonderful life: a career as an academic scientist. Mol Biol Cell 21: 11-14. PMCID: PMC2801704.
  • (pdf) - Yu, C.-H., Wu, H.-J., Kaizuka, Y., Vale, R.D., Groves, J.T. (2010) Altered actin centripetal retrograde flow in physically restricted immunological synapses. PL0S One: 5(7): e11878. PMCID: PMC2912367.
  • (pdf) - Yudushkin, I.A., and Vale, R.D. (2010) Imaging T-cell receptor activation reveals accumulation of tyrosine-phosphorylated CD3{zeta} in the endosomal compartment. Proc Natl Acad Sci USA 107: 22128-22133. PMCID: PMC3009781.


  • (pdf) - Depristo, M.A., Chang, L., Vale, R.D., Khan, S.M., and Lipkow, K. (2009) Introducing simulated cellular architecture to the quantitative analysis of fluorescent microscopy. Prog Biophys Mol Biol. 100: 25–32. PMID: 19628003. PMCID: PMC2805826.
  • (pdf) - Gennerich, A. and Vale, R. D. . (2009) Walking the walk: How kinesin and dynein coordinate their steps. Curr Opinion in Cell Biol 21: 59-67. PMID: 19179063. PMCID: PMC2668149.
  • (pdf) - Kaizuka, Y., Douglass, A.D., Vardhana, S., Dustin, M.L. and Vale, R.D.  (2009) The coreceptor CD2 uses plasma membrane microdomains to transduce signals in T cells. J Cell Biol 185: 521-34. PMID: 19398758. PMCID: PMC2700390.
  • (pdf) - Kardon, J.R., Reck-Peterson, S.L. and Vale, R.D. (2009) Regulation of the processivity and intracellular localization of S. cerevisiae dynein by dynactin. Proc Natl Acad Sci USA 106: 5669-74. PMID: 19293377. PMCID: PMC2657088.
  • (pdf) - Kardon, J.R. and Vale R.D. (2009) Regulators of the cytoplasmic dynein motor. Nature Rev Molec Cell Biol 10: 854-865. PMID: 19935668. NIHMSID 161107.
  • (pdf) - Ou, G. and Vale, R.D. (2009) Molecular signatures of Cell migration in C. elegans Q neuroblasts. J Cell Biol 185: 77-85. PMID: 19349580. PMCID: PMC2700521.
  • (pdf) - Uehara, R., Nozawa, R., Tomioka, A., Petry, S., Vale, R.D., Obuse, C. and Goshima, G. (2009) The augmin complex plays a critical role in spindle microtubule generation for mitotic progression and cytokinesis in human cells. Proc Natl Acad Sci USA 106: 6998-7003. PMID: 19369198. PMCID: PMC2668966.
  • (pdf) - Vale R.D., Dell, K. (2009) The biological sciences in India: Aiming high for the future. J Cell Biol 184: 342-53. (cover) PMID: 19204144. PMCID: PMC2646550.
  • (pdf) - Vale, R. D., Spudich, J. A., and Griffis, E. (2009) Dynamics of myosin, microtubules, and Kinesin-6 at the cortex during cytokinesis in Drosophila S2 cells. J. Cell Biol. 186: 727–738. PMID: 19720876. PMCID: PMC2742194.


  • (pdf) - Carter, A., Garbarino, J.E., Wilson-Kubalek, E.M., Shipley, W.E., Cho, C., Milligan, R.A., Vale, R.D., Gibbons, I.R.  (2008)Structure and functional role of dynein's microtubule binding domain. Science 322: 1691-1695. (Supplemental) PMID: 19074350. PMCID: PMC2663340.
  • (pdf) - Cho, C., Reck-Peterson, S.L., Vale, R.D. (2008) Regulatory ATPase sites of cytoplasmic dynein affect processivity and force generation.  J Biol Chem 283: 25839-25845. PMID: 18650442. PMCID: PMC2533788.
  • (pdf) - Douglass, A. and Vale, R.D. (2008) Single-molecule imaging of fluorescent proteins. Methods Cell Biol 85: 113-25. PMID: 18155461.
  • (pdf) - Goshima, G., Mayer, M., Zhang, N., Stuurman, N. and Vale, R.D. (2008). Augmin: a protein complex required for centrosome-independent microtubule generation within the spindle. J Cell Biol 181: 421-429. PMID 18443220. PMCID: PMC2364697.
  • (pdf) - Guo, Y., Walther, T.C., Rao, M., Stuurman, N., Goshima, G.,Terayama, K., Wong, J.S., Vale, R.D., Walter, P. and Farese, R.V. (2008) Functional genomic screen reveals genes involved in lipid-droplet formation and utilization. Nature 453: 657-61. PMID: 18408709. PMCID: PMC2734507.
  • (pdf) - Roll-Mecak, A. and Vale, R.D. (2008) Structural basis of microtubule severing by the hereditary spastic paraplegia protein spastin. Nature 451: 363 –- 367. PMID: 18202664. PMCID: PMC2882799.
  • (pdf) - Trammel, M.A., Mahoney, N.M., Agard, D.A. and Vale, R.D. (2008) Mob4 Plays a role in spindle focusing in Drosophila S2 cells. J Cell Sci 121: 1284-1292. PMID: 18388316. NIHMSID: 181554.
  • (pdf) - Vale, R.D. (2008) Microscopes for fluorimeters: The era of single molecule measurements. Cell 135: 779-785. Supplemental Material. PMID: 19041739. PMCID: PMC2762818.
  • (pdf) - Yildiz, A., Tomishige, M., Gennerich, A., Vale, R.D. (2008) Intramolecular strain coordinates kinesin stepping behavior along microtubules. Cell 134: 1030-1041. PMID: 18805095. PMCID: PMC2613771.


  • (pdf) - Dustin, M.L., Starr, T., Coombs, D., Majeau, G.R., Meier, W., Hochman, P.S., Douglass, A., Vale, R.D.,  Goldstein, B. and Whitty, A.  (2007) Quantification and modeling of tripartite CD2-, CD58FC Chimera (Alefacept)-, and CD16-mediated Cell Adhesion. J Biol Chem 282: 34748-34757. PMID: 17911103.
  • (pdf) - Gennerich, A., Carter, A.P., Reck-Peterson, S.L. and Vale, R.D. (2007) Force-induced bidirectional stepping of cytoplasmic Dynein. Cell 131: 952-965. PMID: 18045537. PMCID: PMC2851641.
  • (pdf) - Goshima, G., Wollman, R., Goodwin, S., Zhang, N., Scholey, J.M., Vale, R.D. and Stuurman, N. (2007) Genes required for mitotic spindle assembly in Drosophila S2 cells. Science 316: 417-421.(Supplemental) PMID: 17412918. PMCID: PMC2837481.
  • (pdf) - Griffis, E.R., Stuurman, N. and Vale, R.D. (2007) Spindly, a novel protein essential for silencing the spindle assembly checkpoint, recruits dynein to the kinetochore. J Cell Biol 177: 1005-1015. (cover). PMID: 17576797. PMCID: PMC2064361.
  • (pdf) - Kaizuka, Y., Douglass, A.,Varma, R., Dustin, M. and Vale, R.D. (2007) Mechanisms for segregating T cell receptor and adhesion molecules during immunological synapse formation in Jurkat T cell, Proc Natl Acad Sci USA 104: 20296-20301. PMID: 18077330. PMCID: PMC2154425.
  • (pdf) - Mori, T., Vale, R.D. and Tomishige, M. (2007) How kinesin waits between steps. Nature 450: 750-755. PMID: 18004302.
  • (pdf) - Slep, K.C. and Vale, R.D. (2007) Structural basis of microtubule plus end tracking by XMAP215, Clip-170 and EB1. Mol Cell 27: 976-991. PMID: 17889670. PMCID: PMC2052927.
  • (pdf) - Stuurman, N., Amodaj, N. and Vale, R.D. (2007) µManager: Open Source software for light microscope imaging. Microscopy Today 15: 42-43.


  • (pdf) - Imanishi, M., Endres, N.F., Gennerich, A., and R.D. Vale (2006) Autoinhibition regulates the motility of the C-elegans intraflagellar transport motor OSM-3. J Cell Biol 174: 931-937. PMID: 17000874; PMCID: PMC2064385.
  • (pdf) - Endres, N.F., Yoshioka, C., Milligan, R.A. and R. D. Vale. (2006) A lever arm rotation drives motility of the minus-end-directed kinesin, Ncd. Nature 439: 875-878. PMID: 16382238. PMCID: PMC2851630.
  • (pdf) - Mahoney, N.M., Goshima, G., Douglass, A.D., Vale, R.D. (2006) Making microtubules and mitotic spindles in cells without functional centrosomes. Curr Biol 16: 564-569.
  • (pdf) - Pan, X.Y., Ou, G.S., Civelekoglu-Scholey, G., Blacque, O.E., Endres, N.F., Tao, L., Mogilner, A., Leroux, M.R., Vale, R.D.,and J M. Scholey (2006) Mechanism of transport of IFT particles in C-elegans cilia by the concerted action of kinesin-II and OSM-3 motors. J Cell Biol 174: 1035-1045. PMID: 17000880; PMCID: PMC2064394.
  • (pdf) - Reck-Peterson, S.L., Yildiz, A., Carter, A.P., Gennerich, A., Zhang, N., and Vale, R.D. (2006) Single molecule analysis of dynein processivity and stepping behavior.  Cell 126: 335-348. PMID: 16873064. PMCID: PMC2851639.
  • (pdf) - Roll-Mecak, A. and Vale, R.D. (2006) Making more microtubules by severing: a common theme of noncentrosomal microtubule arrays? J Cell Biol 175: 849-851. PMID: 17178905. PMCID: PMC2064694.
  • (pdf) - Tomishige, M., Stuurman, N. and R Vale (2006) Single-molecule observations of neck linker conformational changes in the kinesin motor protein. Nat Struct Molec Biol 13: 887-894.


  • (pdf) - Cheng, L.W., Viala, J.P.M., Stuurman, N., Weidemann, U., Vale, R.D., Portnoy, D.A. (2005) Use of RNA interference in Drosophila S2 cells to identify host pathways controlling compartmentalization of an intracellular pathogen. Proc Natl Acad Sci USA 102: 13646-13651. PMID: 16157870; PMCID: PMC1224656.
  • (pdf) - Dean, S.O., Rogers, S.L, Stuurman, N., Vale, R.D., and Spudich, James A. (2005) Distinct pathways control recruitment and maintenance of myosin II at the cleavage furrow during cytokinesis. Proc Natl Acad Sci USA 102: 13473-13478. PMID: 16174742; PMCID: PMC1200093.
  • (abstract) - Douglass, A.D. and Vale R.D. (2005). Single molecule imaging in living cells by total internal reflection fluorescence microscopy. In: Cell Biology: A Laboratory Handbook, 3rd ed., Elsevier Sci (USA), pp. 129-136.
  • (pdf) - Douglass, A.D. and Vale, R.D. (2005) Single-molecule microscopy reveals plasma membrane microdomains created by protein-protein networks that exclude or trap signaling molecules in T cells. Cell 121: 937-950. (cover). PMID: 15960980. NIHMSID 184810.
  • (pdf) - Dzhindzhev N.S., Rogers, S.L., Vale R.D., Ohkura H. (2005) Distinct mechanisms govern the localisation of Drosophila CLIP-190 to unattached kinetochores and microtubule plus-ends. J Cell Sci 118: 3781-3790. PMID: 16105886. ______________.
  • (pdf) - Gibbons, I.R., Garbarino, J.E., Tan, C.E., Reck-Peterson, S.L., Vale, R.D., and Carter, A.P. (2005) The affinity of the dynein microtubule-binding domain is modulated by the conformation of its coiled-coil stalk. J Biol Chem 280: 23960-23965. PMID: 15826937. PMCID: PMC1464088.
  • (pdf) - Goshima, G., Nédélec, F., and Vale, R.D. (2005) Mechanisms for focusing mitotic spindle poles by minus-end-directed motor proteins. J Cell Biol 171: 229-240. (cover) PMID: 16247025. PMCID: PMC2171195.
  • (pdf) - Goshima, G. and Vale, R.D. (2005) Cell cycle-dependent dynamics and regulation of mitotic kinesins in Drosophila S2 cells. Mol Biol Cell 16: 3896-3907. PMID: 15958489. PMCID: PMC1182325.
  • (pdf) - Goshima, G., Wollman, R., Stuurman, N., Scholey, J.M., Vale, R.D. (2005) Length control of the metaphase spindle. Curr Biol 15: 1979-1988. (cover) PMID: 16303556.
  • (pdf) - Jambhekar, A., McDermott, K., Sorber, K, Shepard, K.A., Vale, R.D., Takizawa, P.A., DeRisi, J.L. (2005) Unbiased selection of localization elements reveals cis-acting determinants of mRNA bud-localization in Saccharomyces cerevisiae. Proc Natl Acad Sci USA 102: 18005-18010. PMID: 16326802; PMCID: PMC1298916.
  • (pdf) - Mennella, V., Rogers, G.C., Rogers, S.L., Buster, D.W., Vale, R.D. and Sharp, D.J. (2005) Functionally distinct kinesin-13 family members cooperate to regulate microtubule dynamics during interphase. Nat Cell Biol 7: 235-245. PMID: 15723056.
  • (pdf) - Roll-Mecak, A. and Vale, R.D. (2005) The Drosophila homologue of the hereditary spastic paraplegia protein, spastin, severs and disassembles microtubules. Curr Biol 15: 650-655. PMID: 15823537.
  • (pdf) - Slep, K.C., Rogers, S.L., Elliott, S.L., Ohkura, H., Kolodziej, P.A. and Vale, R.D. (2005) Structural determinants for EB1-mediated recruitment of APC and spectraplakins to the microtubule plus end. J Cell Biol 168: 587-598. PMID: 15699215; PMCID: PMC2171753.


  • (pdf) - Collins, S.R., Douglass, A.D., Vale, R.D. and Weissman, J.S. (2004) Mechanism of prion propagation: amyloid growth occurs by monomer addition. PLOS Biology 2: 1582-1590 (cover). PMID: 15383837. PMCID: PMC517824.
  • (pdf) - Dell, K.R. and Vale, R.D. (2004) A tribute to Shinya Inoue and innovation in light microscopy. J Cell Biol 165: 21-5. PMID: 15317131. PMCID: PMC2172095.
  • (pdf)- Klopfenstein, D.R. and Vale, R.D. (2004) The lipid binding pleckstrin homology in UNC-104 kinesin is necessary for synaptic vesicle transport in the Caenorhabditis elegans. Mol Biol Cell 15: 3729-3739. (online Cover image for MBC 16, January, 2005; MBC Cover) PMID: 15155810; PMCID: PMC491832.
  • (pdf) - Lawrence C.J., Dawe R.K., Christie K.R., Cleveland D.W., Dawson S.C., Endow S.A., Goldstein L.S., Goodson H.V., Hirokawa N., Howard J., Malmberg R.L., McIntosh J.R., Miki H., Mitchison, T.J. , Okada Y., Reddy A.S., Saxton W.M., Schliwa M., Scholey J.M., Vale R.D., Walczak C.E., Wordeman L. (2004) A standardized kinesin nomenclature. J Cell Biol 167: 19-22. PMID: 15479732. PMCID: PMC2041940.
  • (pdf) - Reck-Peterson, S.L. and Vale, R.D. (2004) Molecular dissection of the roles of nucleotide binding and hydrolysis in dynein's AAA domains in Saccharomyces cerevisiae. Proc Natl Acad Sci USA 101: 1491-1495. PMID: 14755060; PMCID: PMC341754. Also see: PNAS retraction (pdf).
  • (pdf) - Rogers, G.C., Rogers, S.L., Schwimmer, T.A., Ems-McClung, S.C., Walczak, C.E., Vale, R.D., Scholey, J.M. and Sharp, D.J. (2004) Two mitotic kinesins cooperate to drive sister chromatid separation during anaphase. Nature 427: 364-370. PMID: 14681690.
  • (pdf) - Rogers, S.L., Weidemann, U., Haecker, U. and Vale, R.D. (2004) Drosophila DRhoGEF2 associates with microtubule plus ends in an EB1-dependent manner. Curr Biol 14: 1827-1833 (cover). PMID: 15498490.
  • (pdf) - Yildiz, A., Tomishige, M., Vale, R.D. and Selvin, P.R. (2004) Kinesin Walks Hand-Over-Hand. Science 303: 676-678. PMID: 14684828.


  • (pdf) - Al-Bassam, J., Cui, Y., Klopfenstein, D., Carragher, B.O., Vale, R.D. and Milligan, R. A. (2003) Distinct conformations of the kinesin Unc104 neck regulate a monomer-to-dimer motor transition. J Cell Biol 163: 743-753. PMID: 14638858. PMCID: PMC2173678.
  • (pdf) - Goshima, G. and Vale, R.D. (2003) The roles of microtubule-based motor proteins in mitosis: Comprehensive RNAi analysis in the Drosophila S2 cell line. J Cell Biol 162: 1003-1016. (figures)(movies)(cover) PMID: 12975346. PMCID: PMC2172859.
  • (pdf) - Naber, N., Rice, S., Matuska, M., Vale, R.D., Cooke, R., Pate, E. (2003) EPR spectroscopy shows a microtubule-dependent conformational change in the kinesin switch 1 domain. Biophys J 84:3190-6. PMID: 12719248. PMCID: PMC1302879.
  • (pdf) - Naber, N., Minehardt, T.J., Rice, S., Chen, X., Grammer, J., Matuska, M., Vale, R.D., Kollman, P.A., Ca,r R., Yount, R.G., Cooke R., Pate, E. (2003) Closing of the nucleotide pocket of kinesin-family motors upon binding to microtubules. Science 300:798-801. PMID: 12730601.
  • (pdf) - Rice, S., Cui, Y., Sindelar, C., Naber, N., Matuska M., Vale, R. and Cooke R. (2003) Thermodynamic properties of the kinesin neck region docking to the catalytic core. Biophysical J 84:1844-1854. PMID: 12609886. PMCID: PMC1302753.
  • (pdf) - Rogers, S.L., Weidemann, U., Stuurman, N. & Vale, R.D. (2003) Molecular requirements for actin-based lamella formation in Drosophila S2 cells. J Cell Biol 162: 1079-1088 (cover). PMID: 12975351. PMCID: PMC2172842.
  • (pdf) - Rothenberg, M.E., Rogers, S.L., Vale, R.D., Jan, L.Y. and Jan, Y.N. (2003) Drosophila Pod-1 crosslinks both actin and microtubules, and controls the targeting of axons. Neuron 39: 779-791. PMID: 12948445.
  • (pdf) - Shepard, K.A., Gerber, A.P., Jambhekar, A., Takizawa, P.A., Brown, P.O., Herschlag, D., DeRisi, J.L. and Vale, R.D.. (2003) Widespread cytoplasmic mRNA transport in yeast: Identification of 22 bud-localized transcripts using DNA microarray analysis. Proc Natl Acad Sci USA 100: 11429-11434. (supplemental) PMID: 13679573; PMCID: PMC208774.
  • (pdf) - Vale, R.D. (2003) Myosin V motor proteins: marching stepwise towards a mechanims. J Cell Biol 163: 445-450 (cover). PMID: 14610051.
  • (pdf) - Vale, R.D. (2003) The molecular motor toolbox for intracellular transport. Cell 112: 467-480. PMID: 12600311.
  • (pdf) - Yaffe, M.P., Stuurman, N., Vale, R.D. (2003) Mitochondrial positioning in fission yeast is driven by association with dynamic microtubules and mitotic spindle poles. Proc Natl Acad Sci USA 100: 11424-11428. PMID: 12972644; PMCID: PMC208773.


  • (pdf) - Irie, K., Tadauchi, T., Takizawa, P.A.., Vale, R.D., Matsumoto, K. and Herskowitz, H. (2002) The Khd1 protein, which has three KH RNA-binding motifs, is required for proper localization of ASH1 mRNA in yeast. EMBO J. 21: 1158-1167. PMID: 11867544.
  • (pdf) - Klopfenstein, D., Holleran, E.A. and Vale, R.D. (2002) Kinesin motors and microtubule- based organelle transport in Dictyostelium discoideum. J Mus Res Cell Motil 23: 631-638. PMID: 12952062.
  • (pdf) - Klopfenstein, D., Tomishige, M., Stuurman, N. and Vale, R.D. (2002) Role of Phosphatidylinositol(4,5)bisphosphate Organization in Membrane Transport by the Unc104 Kinesin Motor. Cell 109: 347-358 (cover). PMID: 12015984. PMCID: PMC2851634.
  • (pdf) - Rogers, S.L., Rogers, G.C., Sharp, D.J. and Vale, R.D. (2002) Drosophila EB1 is important for proper assembly, dynamics, and positioning of the mitotic spindle. J Cell Biol 158: 873-884 (cover). PMID: 12213835. PMCID: PMC2173155.
  • (pdf) - Tomishige, M., Klopfenstein, D., and Vale, R.D. (2002) Conversion of Unc104/KIF1A kinesin into a processive motor after dimerization. Science 297: 2263-2267. PMID: 12351789.


  • (pdf) - Case, R. B., Rice, S., Hart, C., Ly, B., and Vale, R.D. (2000) Role of the kinesin neck linker and catalytic core in microtubule-based motility. Curr Biol 10: 157- 160 (cover). PMID: 13679573.
  • (pdf) - Dell, K., Turck, C. W., and Vale, R. D. (2000) Mitotic phosphorylation of the dynein light intermediate chain is mediated by cdc2 kinase. Traffic 1: 38-44. PMID: 11208058.
  • (pdf) - Govindan, B. and Vale, R. D. (2000) Characterization of a microtubule assembly inhibitor from Xenopus oocytes. Cell Motil Cytoskel 45: 51-57. PMID: 10618166.
  • (pdf) - Hopkins, S.C., Vale, R.D., and Kuntz, I.D. (2000) Inhibitors of kinesin activity from structure-based computer screening. Biochemistry 39: 2805-2814. PMID: 10704233.
  • (pdf) - Khan, S., Pierce, D.W., and Vale, R. D. (2000) Interactions of the chemotaxis signal protein CheY with bacterial flagellar motors visualized by evanescent wave microscopy. Curr Biol 10: 927-930. PMID: 10959841.
  • (pdf) - Klopfenstein, D., Vale, R.D., and Rogers, S. (2000) Motor protein receptors: moonlighting on other jobs. Cell 103: 537-540. PMID: 11106727.
  • (pdf) - Prahlad, V., Helfand, B.T., Langford, G. M., Vale, R. D., and Goldman, R. D. (2000) Fast transport of neurofilament protein along microtubules in squid axoplasm. J Cell Sci 113: 3939-3946. PMID: 11058081.
  • (pdf) - Shimizu, T., Thorn, K., Ruby, A. and Vale, R.D. (2000) ATPase kinetic characterization and single molecule behavior of mutant human kinesin motors defective in microtubule-based motility. Biochemistry 37: 5265-5273. PMID: 10819995.
  • (pdf) - Takizawa, P.A., DeRisi, J.L., Wilhelm, J. E., and Vale, R. D. (2000) Plasma membrane compartmentalization in yeast by messenger RNA transport and a septin diffusion barrier. Science 290: 341-344. PMID: 11030653.
  • (pdf) - Takizawa, P.A. and Vale, R.D. (2000) The myosin motor, Myo4p, binds Ash1 mRNA via the adapter protein, She3p. Proc Natl Acad Sci USA 97: 5273-5278. PMID: 10792032. PMCID: PMC25818.
  • (abstract) - Thorn, K.S., Naber, N., Matuska, M., Vale, R.D. and Cooke R. (2000) A novel method of affinity-purifying proteins using a bis-arsenical fluorescein. Protein Sci 9: 213-217. PMID: 10716173. PMCID: PMC2144555.
  • (pdf) - Thorn, K.S., Ubersax, J.A., and Vale, R.D. (2000) Engineering the processive run length of the kinesin motor. J Cell Biol 151: 1093-1100 (cover). PMID: 11086010. PMCID: PMC2174356.
  • (pdf) - Tomishige, M. and Vale, R.D. (2000) Controlling kinesin by reversible disulfide cross-linking: identifying the motility-producing conformational change. J Cell Biol 151: 1081-1092 (cover). PMID: 11086009. PMCID: PMC2174365.
  • (pdf) - Vale, R.D. (2000) AAA Proteins: Lords of the ring. J Cell Biol 150: F13-F19. PMID: 10893253. PMCID: PMC2185557.
  • (abstract) - Vale, R. D., Case, R. B., Sablin, E., Hart, C. and Fletterick, R. (2000) Searching for kinesin's mechanical amplifier. Proc Royal Soc London 355: 449-457. PMID: 10836498. PMCID: PMC1692751.
  • (pdf) - Vale, R. D. and Milligan, R. D. (2000). The way things move: looking under the hood of molecular motors. Science 288: 88-95. PMID: 10753125.
  • (pdf) - Wilhelm, J. E., Mansfield, J., Hom-Booher, N., Wang, S., Turck, C., Hazelrigg, T. and Vale, R. D. (2000) Isolation of a ribonucleoprotein complex involved in mRNA localization in Drosophila oocytes. J Cell Biol 148: 427-439. PMID: 10662770. PMCID: PMC2174796.
  • (pdf) - Wilhelm, J. E., Vale, R.D. and Hedge, R.S. (2000) Coordinate control of translation and localization of Vg1 mRNA in Xenopus oocytes. Proc Natl Acad Sci USA 97: 13132-13137. PMID: 11087864. PMCID: PMC27190.


  • (pdf) - Friedman, D. S. and Vale, R. D. (1999) Single molecule analysis of kinesin motility reveals regulation by the cargo-binding tail domain. Nature Cell Biol. 1: 293-297. PMID: 10559942.
  • (pdf) - Hartman, James J. and Vale, R. D. (1999) Microtubule disassembly by ATP-dependent oligomerization of the AAA enzyme katanin. Science 286: 782-785. PMID: 10531065.
  • (pdf) - Pierce, D.W., Hom-Booher, N., Otsuka, A.J. and Vale, R.D. (1999) Single-molecule behavior of monomeric and heteromeric kinesins. Biochem 38: 5412-5421. PMID: 10220328.
  • (abstract) - Pierce, D.W. and Vale, R.D. (1999) Single molecule fluorescence detection of green fluorescence protein and application to single protein dynamics. Meth Cell Biol 58: 49-73. PMID: 9891374.
  • (pdf) - Pollock, N., de Hostos, E.L., Turck, C.W. and Vale, R.D. (1999) Reconstitution of membrane transport powered by a novel dimeric kinesin motor of the Unc104/KIF1A family purified from Dictyostelium. J Cell Biol 147: 493-505 (cover). PMID: 10545495.
  • (pdf) - Rice, S., Lin, A., W., Safer, D., Hart, C. L., Naber, N., Carragher, B. O., Cain, S. M., Pechatnikova, E., Wilson-Kubelek, E. M., Whitaker, M., Pate, E., Cooke R. , Taylor, E. W., Milligan, R. A., and Vale, R. D. (1999) A structural change in the kinesin motor protein that drives motility. Nature 402: 778-783. PMID: 10617199.
  • (pdf) - Sharp, D.J., McDonald, K.L., Brown, H.M., Matthies, H.J., Walczak, C., Vale, R.D, Mitchison, T.J. and Scholey, J.M. (1999) The bipolar kinesin, KLP61F, cross-links microtubules within interpolar microtubule bundles of Drosophila embryonic mitotic spindles. J Cell Biol 144: 125-138. PMID: 9885249.
  • (pdf) - Vale, R. D. (1999) Millennial musings on molecular motors. Trends Cell Biol. 9: M38-M42. PMID: 10611679.


  • (pdf) - de Hostos, E.L., McCaffrey, G., Sucgang, R., Pierce, D.W., and Vale, R. D. (1998) A developmentally regulated kinesin-related motor protein from Dictyostelium discoideum. Mol Biol Cell 9: 2093-2106 (cover). PMID: 9693369.
  • (pdf) - DePace, A.H., Santoso, A., Hillner, P. and Weissman, J.S. (1998) A critical role for amino-terminal glutamine/asparagine repeats in the formation and propagation of a yeast prion. Cell 93: 1241-1252. PMID: 9657156.
  • (pdf) - Hartman, J.J., Mahr, J., McNally, K., Okawa, K., Iwamatsu, A., Thomas, S., Cheesman, S., Jeuser, ., Vale ,R .D. and McNally, F. J. (1998) Katanin, a microtubule-severing protein, is a novel AAA ATPase that targets to the centrosome using a WD40-containing subunit. Cell 93: 277-287. PMID: 9568719.
  • (pdf) - Kull, F. J., Vale, R.D. and Fletterick, R. (1998) The case for a common ancestor: kinesin and myosin motor proteins and G proteins. J Muscle Res Cell Motil 19: 877-886. PMID: 10047987.
  • (pdf) - Lohman, Timothy M., Thorn, Kurt and Vale, Ronald D. (1998) Staying on track: common features of DNA helicases and microtubule motors. Cell 93: 9-12. PMID: 9546385.
  • (abstract) - Pierce, D.W. and Vale, R.D. (1998) Assaying processive movement of kinesin by fluorescence microscopy. Meth Enzymol 298: 154-171. PMID: 9751879.
  • (pdf) - Pollock, N., Koonce, M.P., de Hostos, E.L. and Vale, R.D. (1998) In vitro microtubule-based organelle transport in wild-type Dictyostelium and and cells overexpressing a truncated dynein heavy chain. Cell Motil Cytoskel 40: 304-314. PMID: 9678672.
  • (pdf) - Prahlad, V., Moir, R.D., Vale, R.D. and Goldman, R.D. (1998) Rapid movements of vimentin on microtubule tracks: Kinesin-dependent assembly of intermediate filamenet networks. J Cell Biol 143: 159-170. PMID: 9763428.
  • (pdf) - Romberg, Laura, Pierce, Daniel W., and Vale, Ronald D. (1998) Role of the kinesin neck region in processive microtubule-based motility. J Cell Biol 140: 1407-1416 (cover). PMID: 9508773.
  • (pdf) - Sablin E.P., Case R.B., Dai S.C., Hart C.L., Ruby A., Vale R.D. and Fletterick, R. (1998) Direction determination in the minus-end-directed kinesin motor ncd. Nature 395: 813-816. PMID: 9796817.
  • Wells, S.E., Hillner, P.E., Vale, R.D. and Sachs, A.B. (1998) Circularization of mRNA by eukaryotic translation initiation factors. Mol Cell 2: 135-140. PMID: 9702200.


  • (pdf) - Case, R. B., Pierce, D. W., Hom-Booher, N.; Hart, C. L. and Vale, R. D. (1997) The directional preference of kinesin motors is specified by an element outside of the motor catalytic domain. Cell 90: 959-966. PMID: 9298907.
  • (pdf) - Coppin, Chris M, Pierce, Daniel W., Hsu, Long and Vale, Ronald D. (1997) The load dependence of kinesin's mechanical cycle. Proc Natl Acad Sci USA 94: 8539-44. PMID: 9238012.
  • (pdf) - Funatsu, Takashi, Harada, Yoshie, Higuchi, Hideo, Tokunaga, Makio, Saito, Kiwamu, Ishii, Yoshiharu, Vale, Ronald D. and Yanagida, Toshio. (1997) Imaging and nano-manipulation of single biomolecules. Biophys Chem 68: 63-72. PMID: 9468610.
  • (pdf) - Pierce, Daniel W., Hom-Booher, Nora and Vale, Ronald D. (1997) Imaging individual green fluorescent proteins. Nature 388: 338. PMID: 9237750.
  • (pdf) - Sosa, Hernando, Dias, D. Prabha, Hoenger, Andreas, Whittaker, Michael, Wilson-Kubalek, Elizabeth, Sablin, Elena, Fletterick, R., Vale, Ronald D and Milligan, Ronald A. (1997) A model for the microtubule-Ncd motor protein complex obtained by cryo-electron microscopy and image analysis. Cell 90: 217-224. PMID: 9244296.
  • (pdf) - Takizawa, Peter A., Sil, Anita, Swedlow, Jason R., Herskowitz, Ira and Vale, Ronald D. (1997) Actin-dependent localization of an RNA encoding a cell-fate determinant in yeast. Nature 389: 90-93. PMID: 9288973.
  • (pdf) - Tripet, Brian, Vale, Ronald D. and Hodges, Robert S. (1997) Demonstration of coiled-coil interactions within the kinesin neck region using synthetic peptides. J Biol Chem 272: 8946-56. PMID: 9083016.
  • (pdf) - Vale, Ronald D. and Fletterick, R. (1997) The design plan of kinesin motors. Annu Rev Cell Dev Biol 13: 745-77. PMID: 9442886.
  • (pdf) - Woehlke, Günther, Ruby, Aaron K., Hart, Cynthia L., Ly, Bernice, Hom-Booher, Nora and Vale, Ronald D. (1997) Microtubule interaction site of the kinesin motor. Cell 90: 207-216. PMID: 9244295.


  • (pdf) - Coppin, Chris M., Finer, Jeffrey T., Spudich, James A. and Vale, Ronald D. (1996) Detection of sub-8-nm movements of kinesin by high-resolution optical-trap microscopy. Proc Natl Acad Sci USA 93: 1913-17. PMID: 8700858.
  • (pdf) - Fowler, V.M. and Vale, R.D. (1996) Editorial overview: Cytoskeleton. Curr Opinion in Cell Biol 8: 1-3. PMID: 8791412.
  • (pdf) - Kull, F. Jon, Sablin, Elena P., Lau, Rebecca, Fletterick, R. and Vale, Ronald D. (1996) Crystal structure of the kinesin motor domain reveals a structural similarity to myosin. Nature 380: 550-555 (cover). PMID: 8606779. PMCID: PMC2851642.
  • (pdf) - McNally, Francis J., Okawa, K, Iwamatsu, Akihiro and Vale, Ronald D. (1996) Katanin, the microtubule-severing ATPase, is concentrated in centrosomes. J Cell Sci 109: 561-7. PMID: 8907702.
  • (pdf) - Niclas, J., Allan, Viki J. and Vale, Ronald D. (1996) Cell cycle regulation of dynein association with membranes modulates microtubule-based organelle transport. J Cell Biol 133(3): 585-93. PMID: 8636233. PMCID: PMC2120815.
  • (pdf) - Sablin, Elena P., Kull, F. Jon, Cooke R., Vale, Ronald D. and Fletterick, R. (1996) Crystal structure of the motor domain of the kinesin-related motor ncd. Nature 380: 555-559 (cover). PMID: 8606780.
  • (pdf) - Vale, Ronald D. (1996) Switches, latches and amplifiers: common themes of G proteins and molecular motors (Commentary). J Cell Biol 135: 291-302. PMCID: PMC2121043.
  • (pdf) - Vale, Ronald D., Funatsu, Takashi, Pierce, Daniel W., Romberg, Laura, Harada, Yoshie and Yanagida, Toshio. (1996) Direct observation of single kinesin molecules moving along microtubules. Nature 380: 451-453 (cover). PMID: 8602245. PMCID: PMC2852185.
  • (abstract) - Vignali, G., Niclas, J., Sprocati, M.T., Vale, R.D., Sirtori, C. and Navone, F. (1996) Differential expression of ubiquitous and neuronal kinesin heavy chains during differentiation of human neuroblastoma and PC12 cells. Eur J Neurosci 8: 536-44. PMID: 8963445.
  • (abstract) - Wilhelm, James E. and Vale, Ronald D. (1996) A one-hybrid system for detecting RNA-protein interactions. Genes to Cells 1: 317-23. PMID: 9133665.

Reviews & Chapters

  • (pdf) - Vale RD, Dell K. (2009) The biological sciences in India: Aiming high for the future. J Cell Biol 184: 342-53. (cover) PMID: 19204144. PMCID: PMC2646550.
  • (pdf) - Vale, R.D. (2008) Microscopes for Fluorimeters: The Era of Single Molecule Measurements . Cell 135: 779-785.
  • (abstract) - Douglass, A.D. and Vale R.D. (2005). Single molecule imaging in living cells by total internal reflection fluorescence microscopy. In: Cell Biology: A Laboratory Handbook, 3rd ed., Elsevier Sci (USA), pp. 129-136.
  • (pdf) - Klopfenstein, D., Vale, R.D., and Rogers, S. (2000) Motor protein receptors: moonlighting on other jobs. Cell 103: 537-540.
  • (pdf) - Vale, R.D. (2000) AAA Proteins: Lords of the ring. J Cell Biol 150: F13-F19
  • (abstract) - Vale, R. D., Case, R. B., Sablin, E., Hart, C. and Fletterick, R. (2000) Searching for kinesin's mechanical amplifier. Proc Royal Soc London 355: 449-457.
  • (pdf) - Vale, R.D., Milligan, R.A. (2000) The way things move: looking under the hood of molecular motor proteins. Science 288: 88-95
  • (pdf) - Vale, R. D. (1999) Millennial musings on molecular motors. Trends Cell Biol 9: M38-M42.
  • (abstract) - Pierce, D.W. and Vale, R.D. (1998) Detection of single molecule GFP fluorescence. Meth Enzymol 298: 154-171
  • (pdf) - Vale, Ronald D. and Fletterick, R. (1997) The design plan of kinesin motors. Annu Rev Cell Dev Biol 13: 745-77.
  • (pdf) - Vale, R.D., Reese, T.S. and Sheetz, M.P. (1985) Identification of a novel force generating protein, kinesin, involved in microtubule-based motility. Cell 42: 39-50. NIHMSID 184995.
  • (pdf) - Vale, Ronald D. (1996) Switches, latches and amplifiers: common themes of G proteins and molecular motors (Commentary). J Cell Biol 135: 291-302.

Publications pre-1995