Microtubules and Mitosis Movies and Images
These movies and images can be used for teaching purposes. Please acknowledge the source as the Vale Lab web site or iBioSeminars.Org.
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|iBio Seminar Clip: How are spindle microtubules made? (5:35)||
iBio Seminar Clip: Spindle formation: Image of a metaphase spindle and video of first steps in spindle formation. (1:00)
|Rhodamine-labeled microtubules being severed by katanin.||EB1-GFP in mitosis. Time-lapse observation of EB1-GFP in mitosis. EB1-GFP is accumulated at the growing tips of microtubules. Images were taken every 1 sec using spinning-disk confocal microscopy.|
|GFP-tubulin in mitosis movie 1 (wt). Time-lapse observation of GFP-tubulin in wild-type S2 cells during bipolar spindle formation. Images are taken every 10 sec using spinning-disk confocal microscopy.||GFP-tubulin in mitosis movie (klp10A RNAi). Time-lapse observation of GFP-tubulin in S2 cells after Klp10A [Kin I] RNAi. Monopolar spindle is initially formed, but is converted to bipolar spindle through acentrosomal pole formation.|
|S2 Mitosis: S2 cells expressing GFP-H2B, Cherry tubulin, z-stacks of 8 slices. One z-stack every 10 seconds. Movie is a maximum intensity projection. Movie by Nico Stuurman.|
(Click images for larger)
|Patronin: Artistic depiction of Patronin. Because of ssp4’s protective role, we named the protein “Patronin” from the protective patronus in the Harry Potter novels (Harry Potter’s patronus was a stag, and hence the stag protecting the microtubule minus end in Graham Johnson’s illustration shown here (firstname.lastname@example.org).||Spastin: Through human genetic studies to find genetic mutations that underlie spastic parapalegias, the gene spastin was identified and found to be closely related to katanin.|
|Spindle phenotypes: Different cagetories of phenotypes produced aftere RNAi gene knockdown in Drosophila S2 cells.||Anaphase in Drosophila S2 cell.|
Model for spastin mediated microtubule disassembly.
|A model for augmin-gamma-tubulin mediated microtubule nucleantion.|
|Patronin bound to microtubule minus ends in vitro.|
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|last updated 9/20/12|